Human Ramucirumab (Ramucirumab) ELISA Kit

Cat.#:

JL58218

Regulatory Status:

RUO

Alternative:

Ramucirumab

Detection Method:

ELISA

Assay Type:

Indirect

Detection Range:

0.78-50ng/mL

Sensitivity:

0.37ng/mL

Specificity:

This kit recognizes Human Ramucirumab in samples. No significant cross-reactivity or interference between Human Ramucirumab and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Cancer

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| Ramucirumab

Ramucirumab is a fully human monoclonal antibody (IgG1) used for the treatment of cancer.Ramucirumab is a human vascular endothelial growth factor receptor 2 (VEGFR2) antagonist.Ramucirumab was developed by ImClone Systems.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	50	25	12.5	6.25	3.12	1.56	0.78	0
OD	2.72	1.63	0.9	0.54	0.35	0.27	0.22	0.08
Corrected OD	2.64	1.55	0.82	0.46	0.27	0.19	0.14	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of HumanRamucirumab spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	92
Plasma(n=8)	92-105	96
Cell Culture Supernates(n=8)	95-110	99

| Linearity

Samples were spiked with high concentrations of HumanRamucirumab and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-98	88-96	90-110
1 : 2	Average (%)	92	92	95
1 : 4	Range (%)	89-102	87-108	105-115
1 : 4	Average (%)	90	95	105